Eye Sigil ARG/Choice Chamber
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Choice Chamber is a real-time procedurally generated game developed by Studio Bean that is "crowdsourced", i.e. the progression in the game is tied to people writing keywords in the connected Twitch chat. The game was released on 16 Jul 2015.
The sigil was added in an update 16 Sep 2016.
Sigil
Obtaining the glitch heart in room 8 triggered the sigil to appear ingame and to pop up an error message that included "say nothing".
Piece
Saying "nothing" in a linked twitch chat while in room 8 caused the chat bot to emit a reversed imgur url. That url was http://imgur.com/iIAI6ah. The image, also mirrored, was cryptically asking you to go back 501 rooms from the beginning and into room -500. Once there you needed to wait about an hour while the map piece assembled itself.
Data Mining
The sigil is stored in the image BitsLossless2 (801) of the game's SWF.